The term “biofilm” refers to the. 7. attachment of microorganisms on surface and formation of aggregates in a self produced polymeric matrix (11) commonly known as extracellular polymeric substances (EPS) which consists of polysaccharides, proteins, glycoproteins, glycolipids and extracellular DNA (12) A well-structured work that Phd Thesis Biofilm includes such sections as an abstract, introduction, materials and methods, results, discussion and literature cited. A list of credible sources. Phd Thesis Biofilm Our writers use EBSCO to access peer-reviewed and up-to-date materials. If you have a list of required sources handy, feel free to Rational design of biomaterials is hindered by the lack of quantitative structure-activity relationships (QSAR). Here we report the use of a QSAR to guide experimentation into a new chemical space; we predict and synthesize molecular structures for novel monomers that will reduce biofilm formation, and so identifying the lowest biofilm attachment polymer discovered to date
Items in DSpace are phd thesis biofilm by copyright, with all rights reserved, unless otherwise indicated. Newcastle University eTheses Newcastle University Faculty of Science, Agriculture and Engineering School of Chemical Engineering and Advanced Materials, phd thesis biofilm.
Show full item record. A study on the diversity and production of microbial extracellular nucleases :potential anti-biofilm enzymes. Rajarajan, Nithyalakshmy. Biofilms are beneficial for organisms to survive in natural environment as well as for biotechnological applications such as microbial fuel cells and bioremediation. However, phd thesis biofilm, biofilms are associated with disease persistence and biofouling and are comprised of adhered microbes within a hydrated matrix rich in polysaccharides, proteins and extracellular DNA eDNA, phd thesis biofilm.
eDNA is an important structural component and its degradation by deoxyribonucleases may be a novel approach to eradicate biofilm related problems. The present work was undertaken in this context to discover and produce microbial nucleolytic enzymes for applications for the control of harmful biofilms.
Eighty six out phd thesis biofilm bacterial isolates which included thermophilic and psychrophilic strains, showed deoxyribonuclease activity.
The diversity and function of extracellular nucleases was also investigated throughout the microbial world using bioinformatics tools. Sequence driven analysis suggested that major bacterial lineages contain diverse extracellular nucleases with biological function related to nitrogen, phosphate and carbohydrate metabolism, protection, survival and virulence.
Production optimisation for one specific extracellular nuclease, NucB, from Bacillus licheniformis EI was carried out. This enzyme was previously known to cleave eDNA causing biofilm dispersal, and may therefore be used commercially to remove biofilms. The understanding of B. licheniformis physiology was applied in order to enhance NucB production fold. For phd thesis biofilm characterisation of the enzyme and to Abstract iv understand its biological mechanism in breaking down biofilms, NucB was expressed in the SURE expression host B.
subtilis NZ This allowed a fold increase in protein yield. Biophysical characterisation showed that the protein was thermally stable and could reversibly refold. Statistical optimisation of extracellular nucleases production in diverse bacteria grown at different temperatures was demonstrated as a promising methodology for enhancing key enzyme secretion. The effectiveness of biofilm disruption by NucB was successfully tested with different single species biofilms grown on polystyrene, glass, and stainless steel surfaces.
The results presented in this thesis demonstrate that bacteria were able to produce nucleases across broad temperature range. In context to biofilm dispersal, bioinformatic analysis speculates the ecological implication of secreted diverse microbial extracellular nuclease-like genes were to decide the fate of eDNA and play pivotal role in nutrient cycling of the eco-system.
Bioprocess development confirmed process optimisation can reliably produce functional and well-folded recombinant NucB at levels suitable for applications where biofilm removal is needed. Production optimisation of extracellular nucleases from diverse bacteria expanded the availability of different nucleases with wide range of anti-biofilm properties.
Evidence is also presented to show that extracellular nucleases can disperse preformed microbial biofilms on different substrata. Microbial extracellular nucleases therefore appear to be a rich and unexplored source of anti-biofilm enzymes. School of Chemical Engineering and Advanced Materials. Rajarajan, phd thesis biofilm, N.
Submitting my PhD Thesis
, time: 22:12
The term “biofilm” refers to the. 7. attachment of microorganisms on surface and formation of aggregates in a self produced polymeric matrix (11) commonly known as extracellular polymeric substances (EPS) which consists of polysaccharides, proteins, glycoproteins, glycolipids and extracellular DNA (12) Phd Thesis Biofilm Give us your assignments and a subject matter expert will get it done quickly and painlessly. Better grades can be yours without stress!/10() Studying biofilm dynamics and immunomodulation is crucial in understanding the pathogenicity of this opportunistic pathogen. Biofilm formation is a mechanism adopted by most organisms at interphases and is the primary mode of survival
No comments:
Post a Comment